Construction, Cloning, and Overexpression of Staphylococcal Enterotoxin B Gene Synthetic (SEBsyn) in pET-28a(+): Pre-development Bacterial-Toxin Therapy for Cancer

Lysosomal catabolism of a protein toxin: staphylococcal enterotoxin B.

Molecular cloning of staphylococcal enterotoxin B gene in Escherichia coli and Staphylococcus aureus.

We have cloned the Staphylococcus aureus entB gene in Escherichia coli, using pBR322 as the vector plasmid; however, no detectable staphylococcal enterotoxin B (SEB) was produced by the E. coli clones. When the entB gene was placed downstream from the strong lambda phage promoter, PR, SEB was synthesized at readily detectable levels in E. coli. Interestingly, mature SEB was almost exclusively p...

molecular screening of staphylococcal enterotoxin b gene in clinical isolates

objective: the role of staphylococcal enterotoxin b (seb) in food poisoning is well known, however its role in other diseases remains to be explored. the aim of this study is the molecular screening and characterization of the seb gene in clinically isolated strains. materials and methods: in this experimentally study, 300 staphylococcus aureus (s. aureus) strains isolated from clinical samples...

Molecular Screening of Staphylococcal Enterotoxin B Gene in Clinical Isolates

OBJECTIVE The role of staphylococcal enterotoxin B (SEB) in food poisoning is well known, however its role in other diseases remains to be explored. The aim of this study is the molecular screening and characterization of the SEB gene in clinically isolated strains. MATERIALS AND METHODS In this experimentally study, 300 Staphylococcus aureus (S. aureus) strains isolated from clinical samples...

Protection against bacterial superantigen staphylococcal enterotoxin B by passive vaccination.

We investigated the ability of two overlapping fragments of staphylococcal enterotoxin B (SEB), which encompass the whole toxin, to induce protection and also examined if passive transfer of chicken anti-SEB antibodies raised against the holotoxin could protect rhesus monkeys against aerosolized SEB. Although both fragments of SEB were highly immunogenic, the fragments failed to protect mice wh...